Comparative Evaluation of Rapid Antigen Detection with Reverse Transcriptase Polymerase Chain Reaction for Detection of Novel SARS-CoV-2.

  • Qadri U Sher-i-kashmir Institute of Medical Scienc es
  • Fomda BA Prof & Head, Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences Soura
  • Bali N Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences Soura, Srinagar, Jammu and Kashmir
  • Wani SJ Department of General Medicine, Sher-i-Kashmir Institute of Medical Sciences Soura, Srinagar, Jammu and Kashmir
Keywords: SARS CoV-2, RTPCR, RADT, Pandemic, Point of care

Abstract

Introduction: Swift and precise detection of SARS-CoV-2 is essential for managing outbreaks both within communities and hospitals. Real-time reverse transcriptase polymerase chain reaction (rRT–PCR) stands as the benchmark diagnostic test for SARS-CoV-2. However, its reliance on specialized equipment and technical expertise, alongside the necessity for a sophisticated laboratory, limits its widespread use. Rapid antigen tests have emerged as convenient point-of-care diagnostic assays. Evaluating the diagnostic accuracy of these tests compared to RT-PCR is crucial. While numerous studies have been conducted for this purpose globally, many have assessed performance using separate samples, potentially leading to variations in findings. Aim: In our study, we aimed to comparatively assess rRT-PCR and Rapid Antigen Tests, with rRT-PCR considered the gold standard, by conducting both tests using samples collected in the same Viral Transport Medium (VTM)
tube. Materials and Methods: We collected a total of 300 nasopharyngeal/oropharyngeal swabs from patients suspected of having COVID-19. Rapid antigen tests were performed directly from the tube using the STANDARD Q COVID-19 Ag test. RT-PCR of the sample was conducted post RNA extraction. Both tests were performed using the same VTM tube.Results: The rapid antigen detection test (RADT) demonstrated a sensitivity and specificity of 86% and 90%, respectively. The positive predictive value (PPV) and negative predictive value (NPV) of RADT were 91% and 88%, respectively. Conclusion: RADT conducted directly from VTM exhibited high sensitivity and specificity, suggesting its potential utility during pandemics.

Keywords: SARS CoV-2, RTPCR, RADT, Pandemic, Point of care

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How to Cite
Qadri, U., Fomda, B. A., Bali, N., & Wani, S. J. (2024). Comparative Evaluation of Rapid Antigen Detection with Reverse Transcriptase Polymerase Chain Reaction for Detection of Novel SARS-CoV-2. Tropical Journal of Pathology and Microbiology, 10(03), 45-50. Retrieved from https://pathology.medresearch.in/index.php/jopm/article/view/657
Issue
Vol 10 No 03 (2024): Jul-Sep
Section
Original Article

Copyright (c) 2024 Author (s). Published by Siddharth Health Research and Social Welfare Society

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