Tropical Journal of Pathology and Microbiology <p><em><strong>ISSN: <a href="" target="_blank" rel="noopener">2456-1487 (Online)</a>, <a href="" target="_blank" rel="noopener">2456-9887 (Print)</a></strong></em></p> <p><em><strong>RNI: MPENG/2017/70771</strong></em></p> Siddharth Health Research and Social Welfare Society en-US Tropical Journal of Pathology and Microbiology 2456-9887 Inducible clindamycin resistance among clinically significant staphylococcus aureus isolates in a tertiary care centre <p><strong>INDUCIBLE CLINDAMYCIN RESISTANCE AMONG CLINICALLY SIGNIFICANT <em>STAPHYLOCOCCUS AUREUS</em> ISOLATES IN A TERTIARY CARE CENTRE </strong></p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </strong><strong><u>ABSTRACT</u></strong></p> <p><strong><u>Objective</u></strong></p> <p>&nbsp;<em>Staphylococcus aureus</em> isolates with inducible clindamycin resistance may lead to&nbsp; therapeutic failure on treating with Clindamycin. Aim:</p> <p>To detect inducible clindamycin resistance of clinically significant <em>Staphylococcus aureus</em>&nbsp; isolates and MIC of clindamycin in them.</p> <p><strong><u>Study design</u></strong></p> <p>Crossectional study</p> <p><strong><u>Study subjects</u></strong></p> <p>The study was conducted in 200 clinically significant <em>Staphylococcus aureus</em> isolates from Govt medical college, Thrissur Kerala, India&nbsp; over a period of one year</p> <p><strong><u>Study methods</u></strong></p> <p>D test was done and &nbsp;iMLS<sub>B</sub>, cMLS<sub>B</sub>, MS phenotypes were identified. Epsilometer test was done for determining the MIC of Clindamycin.. Statistical analysis was done using IBM statistical package for social sciences version 25(SPSS).</p> <p><strong><u>Results </u></strong></p> <p>Of the total&nbsp; 200 samples 132(66%) were MSSA and 68(34%) were MRSA.MS phenotypes 60(30%), iMLS<sub>B</sub> phenotype 53(26.5%),cMLS<sub>B </sub>phenotype 44 (22%), Erythromycin and Clindamycin sensitive strains 41(20.5%) and resistant strains 2 (1%). Inducible and constitutive resistance to clindamycin were more in MRSA.92% of iMLS<sub>B</sub> phenotypes &nbsp;and 96% of MS phenotypes had MIC &lt; 0.5(sensitive). 8% iMLS<sub>B</sub> phenotypes and 96% of cMLS<sub>B </sub>phenotypes had MIC &gt;4(resistant). One isolate with&nbsp; cMLS<sub>B </sub>phenotype&nbsp; had MIC in intermediate range .</p> <p><strong><u>Conclusion</u></strong></p> <p>Prevalence of inducible clindamycin resistance in our hospital is high and more common among MRSA. E test helped to determine MIC in intermediate range (shown by&nbsp; cMLS<sub>B </sub>phenotype )which is not possible by doing disc diffusion method alone. &nbsp;Most common phenotype isolated was MS phenotype.</p> <p><strong><u>Keywords</u></strong></p> <p><strong>iMLS<sub>B</sub>, cMLS<sub>B</sub>, MS Phenotype, D test, MRSA, MSSA, MIC</strong></p> <p><strong><sub>&nbsp;</sub></strong></p> <p><strong><sub>&nbsp;</sub></strong></p> <p><strong><sub>&nbsp;</sub></strong></p> <p><strong><sub>&nbsp;</sub></strong></p> <p><strong><sub>&nbsp;</sub></strong></p> <p>&nbsp;</p> NISHA SADANANDAN Copyright (c) 2024 Author (s). Published by Siddharth Health Research and Social Welfare Society 2024-01-30 2024-01-30 9 4 32 39 Incidental Finding of Microfilaria in Submental Lymph Node Cytology: A Rare Case <p><span style="font-size: 10.0pt;">Filariasis is a worldwide public health concern. It is frequently examined in peripheral smears obtained from overnight sample collection. In cytology smears, microfilariae are rarely detected, despite their considerable incidence. It is rare for a lymph node to appear as a filarial nodule. Furthermore, it is unusual for lymph node fine needle aspiration cytology (FNAC) to reveal microfilariae. We would like to share the instance of a patient who had submental lymph node swelling and incidentally found microfilariae. For 10–15 days, the patient had a 2 x 2 cm enlargement of the submental lymph node along with a complaint of weight loss. Enlarged lymph nodes in the submental area were seen in the USG-neck region. Her condition was tentatively identified as lymph node swelling that was still being assessed. However, microfilariae were unintentionally discovered during the FNAC of the lymph node. When filariasis does not exhibit any clinical symptoms, FNAC can help identify microfilariae in the lymph node.</span></p> Vaidehi Nagar Vibha Patel Sharad Gor Copyright (c) 2024 Author (s). Published by Siddharth Health Research and Social Welfare Society 2024-01-30 2024-01-30 9 4 40 43