Inducible clindamycin resistance among clinically significant staphylococcus aureus isolates in a tertiary care centre

2024-01-30T19:21:39+0530

INDUCIBLE CLINDAMYCIN RESISTANCE AMONG CLINICALLY SIGNIFICANT STAPHYLOCOCCUS AUREUS ISOLATES IN A TERTIARY CARE CENTRE

ABSTRACT

Objective

Staphylococcus aureus isolates with inducible clindamycin resistance may lead to therapeutic failure on treating with Clindamycin. Aim:

To detect inducible clindamycin resistance of clinically significant Staphylococcus aureus isolates and MIC of clindamycin in them.

Study design

Crossectional study

Study subjects

The study was conducted in 200 clinically significant Staphylococcus aureus isolates from Govt medical college, Thrissur Kerala, India over a period of one year

Study methods

D test was done and iMLS_B, cMLS_B, MS phenotypes were identified. Epsilometer test was done for determining the MIC of Clindamycin.. Statistical analysis was done using IBM statistical package for social sciences version 25(SPSS).

Results

Of the total 200 samples 132(66%) were MSSA and 68(34%) were MRSA.MS phenotypes 60(30%), iMLS_B phenotype 53(26.5%),cMLS_Bphenotype 44 (22%), Erythromycin and Clindamycin sensitive strains 41(20.5%) and resistant strains 2 (1%). Inducible and constitutive resistance to clindamycin were more in MRSA.92% of iMLS_B phenotypes and 96% of MS phenotypes had MIC < 0.5(sensitive). 8% iMLS_B phenotypes and 96% of cMLS_Bphenotypes had MIC >4(resistant). One isolate with cMLS_Bphenotype had MIC in intermediate range .

Conclusion

Prevalence of inducible clindamycin resistance in our hospital is high and more common among MRSA. E test helped to determine MIC in intermediate range (shown by cMLS_Bphenotype )which is not possible by doing disc diffusion method alone. Most common phenotype isolated was MS phenotype.

Keywords

iMLS_B, cMLS_B, MS Phenotype, D test, MRSA, MSSA, MIC

Incidental Finding of Microfilaria in Submental Lymph Node Cytology: A Rare Case

2024-01-30T19:22:42+0530

Filariasis is a worldwide public health concern. It is frequently examined in peripheral smears obtained from overnight sample collection. In cytology smears, microfilariae are rarely detected, despite their considerable incidence. It is rare for a lymph node to appear as a filarial nodule. Furthermore, it is unusual for lymph node fine needle aspiration cytology (FNAC) to reveal microfilariae. We would like to share the instance of a patient who had submental lymph node swelling and incidentally found microfilariae. For 10–15 days, the patient had a 2 x 2 cm enlargement of the submental lymph node along with a complaint of weight loss. Enlarged lymph nodes in the submental area were seen in the USG-neck region. Her condition was tentatively identified as lymph node swelling that was still being assessed. However, microfilariae were unintentionally discovered during the FNAC of the lymph node. When filariasis does not exhibit any clinical symptoms, FNAC can help identify microfilariae in the lymph node.

Tropical Journal of Pathology and Microbiology

Inducible clindamycin resistance among clinically significant staphylococcus aureus isolates in a tertiary care centre

Incidental Finding of Microfilaria in Submental Lymph Node Cytology: A Rare Case