Tropical Journal of Pathology and Microbiology https://pathology.medresearch.in/index.php/jopm <p><em><strong>ISSN: <a href="https://portal.issn.org/resource/ISSN/2456-1487" target="_blank" rel="noopener">2456-1487 (Online)</a>, <a href="https://portal.issn.org/resource/ISSN/2456-9887" target="_blank" rel="noopener">2456-9887 (Print)</a></strong></em></p> <p><em><strong>RNI: MPENG/2017/70771</strong></em></p> en-US editor@pathology.medresearch.in (Dr D Sharad Gedam) editor.jopm@medresearch.in (Dr. Sharad Gedam, Mob: 8989622793 (10 AM to 5 PM, Mon- Sat)) Sat, 15 Jan 2022 21:52:54 +0530 OJS 3.1.2.1 http://blogs.law.harvard.edu/tech/rss 60 A study on evaluation of Escherichia coli isolates in urinary tract infection and its antibiogram in view of Emerging drug resistance at a tertiary care hospital https://pathology.medresearch.in/index.php/jopm/article/view/587 <p>Introduction: Urinary tract infection is a common problem in routine clinical practices despite ageand sex, and it is also the most common nosocomial infection. The most common uropathogen isEscherichia coli worldwide.</p> <p>Material and methods: A retrospective study was conducted fromJanuary 2020 to December 2020 in a tertiary-care hospital in South India. All positive urinarycultures with significant bacteriuria received from Inpatient and outpatient wards of variousdepartments were included in the study. The prevalence percentage of different bacteria wasstudied. Multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan drug-resistant (PDR)Escherichia coli in different baseline and demographic characteristics were evaluated.</p> <p>Results: Atotal of 2144 urine samples were processed for culture and sensitivity, out of which 531 sampleswere found to be culture-positive with significant bacteuria. Among 531 samples, 202 (38%) culturepositives were due to Escherichia coli infection. Women (62%) were more susceptible to infection.The predominant age group affected among women was 21-30 yrs and men 51-60 years. Of these,28% exhibited an MDR pattern, 18% XDR and 1.9% PDR.</p> <p>Conclusion: Treatment of Urinary tractinfection due to E. coli may be difficult due to high resistance to commonly used antibiotics. Socontinuous surveillance of drug-resistant organisms and evaluation of drug resistance patterns areneeded to prevent treatment failure and to reduce the economic burden due to these resistantisolates.</p> Mahamad Wajid, Saranya Mallamgunta, V. Prathyusha Pedapati, Shazia Naaz Copyright (c) 2022 Author (s). Published by Siddharth Health Research and Social Welfare Society https://creativecommons.org/licenses/by/4.0 https://pathology.medresearch.in/index.php/jopm/article/view/587 Mon, 17 Jan 2022 00:00:00 +0530 Relationship between blood group and risk of infection, intubation and death in Covid pandemic https://pathology.medresearch.in/index.php/jopm/article/view/582 <p><strong>Background : </strong>Few people infected by the coronavirus become seriously ill, while others show little to no signs of the symptoms or are asymptomatic. Recent researches are pointing to the fact that the ABO blood group might play an important role in a person’s susceptibility and severity of COVID-19 infection.</p> <p><strong>Aims :</strong> This study is conducted to find relationship between ABO groups and COVID-19 infection.</p> <p><strong>Methods : </strong>A total of 395 patients were included in this study. The study population was divided on the basis of ABO blood groups into types A+, A−, B+, B −, AB, O+, and O−.</p> <p><strong>Results : </strong>Blood group B was associated with high susceptibility of infection. We found slightly increased infection prevalence among Rh factor positive blood group. Risk of infection was decreased among blood groups with Rh factor negative. Cases having blood group B have higher chances of covid-19 infection followed by AB and type O.</p> <p><strong>Conclusion : </strong>We estimated Rh-negative blood types to have a protective effect for covid -19 infection. Our results add to the growing body of evidence suggesting blood group type may play a role in spreading COVID-19 infection.</p> <p><strong>Keywords :</strong> COVID-19, ABO blood groups, Severity, Infection</p> Renu Singh; M Siddiqui Copyright (c) 2022 Author (s). Published by Siddharth Health Research and Social Welfare Society https://creativecommons.org/licenses/by/4.0 https://pathology.medresearch.in/index.php/jopm/article/view/582 Tue, 18 Jan 2022 00:00:00 +0530 A cytomorphological study of spectrum of lymphnode lesions in a tertiary care centre – retrospective study https://pathology.medresearch.in/index.php/jopm/article/view/590 <p><strong>Introduction: </strong>Lymphadenopathy (LA) is a clinical condition where the lymph nodes (LNs) can be enlarged. Fine Needle Aspiration Cytology (FNAC) is a simple, inexpensive and a rapid diagnostic technique practiced to find the cause for the superficial LA. With this, a study was conducted to find the cytomorphological features of the enlarged LNs and also to analyze the utility of FNAC in the suspected malignant lesions.</p> <p><strong>Materials and methods: </strong>Study was conducted in the department of Pathology, Kamineni Institute of Medical Sciences, Narketpally from June 2018 to Dec 2020. Random sampling was considered. Individuals of all age groups with enlarged LNs were included.&nbsp; Inadequate or hemorrhagic samples, non cooperative members were not considered. The participants were proceeded for FNAC as per the standard reports. Smear preparation was done at bedside, fixed in 95% Ethyl alcohol. The smears were stained with H &amp; E, Giemsa techniques. If required, Ziehl Neelsen (ZN) technique was also used to find acid fast bacilli (AFB). After staining, the smears were air dried and examined under microscope.</p> <p><strong>Results: &nbsp;</strong>Total 161 (100%) participants were included, age was ranged between 8 months to 85 years and the mean age was 32.7 years. Gender wise, 89 (55.3%) were male and 72 (44.7%) were female participants. Cervical region was identified to be the most common (108; 67%), followed by submandibular and inguinal. &nbsp;Among the TB LA, 8 (16%) were diagnosed to be group 1, 30 (60%) were group 2 and 12 (24%) were group 3 and. Out of the 22 (13.7%) malignant cases, metastasis was seen in 15 (10.55%) and lymphoma in 7 (4.34%).</p> <p><strong>Conclusion: </strong>FNAC precludes the need for biopsy or surgery, saves time and patient resources. Hence FNAC can be considered as an essential diagnostic tool in a clinical set up.</p> Hafsa Saleem, Rida Fatima, Afroze Shameem, T Jaya Chandra, Khatija Shameem Copyright (c) 2022 Author (s). Published by Siddharth Health Research and Social Welfare Society https://creativecommons.org/licenses/by/4.0 https://pathology.medresearch.in/index.php/jopm/article/view/590 Tue, 18 Jan 2022 00:00:00 +0530 A prospective report on histopathological study of ovarian tumor in a tertiary care center https://pathology.medresearch.in/index.php/jopm/article/view/581 <p><strong>Introduction:</strong> Ovarian tumors (OTs) are the most notorious of all gynecological lesions in the reproductive age group. With this a study was conducted to categorize the ovarian lesions into benign, borderline and malignant lesions and also to study their laterality, gross and microscopic patterns.</p> <p><strong>Settings: </strong>Study was conducted in the department of Pathology, KIMS, Narketpally, Telangana from January 2017 to December 2019, 36 months. Random sampling was considered. Women aged <u>&gt;</u>18yrs, histologically proven OTs, sized&nbsp;<u>&gt;</u> 5 cm diameter were included. Women with follicular cysts, haemorrhagic inclusion cysts, endometriosis, those who were non cooperative and didn’t submit the consent were not considered. Abdominal hysterectomy specimen were considered. Gross examination was done. The features such as size, color of specimen were noted. As a part of the study, the external surface features and&nbsp;contents were noted and also recorded in the proforma. After 24 to 48 hrs&nbsp;of fixation, multiple bits were taken from the representative areas which may be prone to be OT. The stained sections were examined under the light microscope for histopathological diagnosis.</p> <p><strong>Results: </strong>Out of 94 participants, 79 were benign tumors (Bet), 5 were borderline tumor (BoT) and 10 were malignant tumor (MlT). Among the BeT, maximum were diagnosed in 21 – 40 years group, 21 – 40 years group in BoT and 41 – 60 years for MlT. In this research, 91% were unilateral and Seromucinouscystadenoma was the common bilateral tumor.&nbsp; Among the different histological patterns, surface epithelial tumors constituted the majority.</p> <p><strong>Conclusion: </strong>Maximum number of tumor cases were benign, reported in the reproductive age group whereas the malignant neoplasms in &gt; 40 yrs. The present study emphasizes the need of proper histopathological evaluation and screening at all ages due to relative predominance of OTs to rule out malignancies.</p> Dr. T Jaya Chandra Copyright (c) 2022 Author (s). Published by Siddharth Health Research and Social Welfare Society https://creativecommons.org/licenses/by/4.0 https://pathology.medresearch.in/index.php/jopm/article/view/581 Thu, 20 Jan 2022 12:13:55 +0530 Histological Variants and Tumor Heterogeneity in Colorectal Cancer https://pathology.medresearch.in/index.php/jopm/article/view/583 <p><strong><em>ABSTRACT:</em></strong></p> <p><strong><em>OBJECTIVE: </em></strong></p> <p>Colorectal cancer is not one disease but rather a collection of neoplastic diseases. Due to heterogeneity in the disease&nbsp; definite categorization, TNM staging as well as different therapy response and prognosis extensive disease stratification is required.</p> <p>Therefore TNM Staging ,Microsatellite stability status ,Tumor Grade, Lymphovascular emboli&nbsp; Intraepithelial lymphocytes, Tumor budding and other parameters are assessed in the pathology report to indicate the extent and prognosis of disease.</p> <p><strong><em>DESIGN AND SUBJECT: </em></strong></p> <p>This is a retrospective study which includes 200 resected specimens of Colorectal Cancer cases.</p> <p>The slides were retrieved and were reviewed together by two pathologists. The results were</p> <p>recorded</p> <p>&nbsp;</p> <p><strong><em>METHODS: </em></strong></p> <p>The histological parameters of 200 Colorectal Cancer cases (which includes right colon, Left Colon ,Rectum and both Left and Right Colon ) &nbsp;without prior radiotherapy and chemotherapy were considered.</p> <p>All radical specimens of Colorectal cancers were fixed in formalin and were subjected to processing and staining by Hematoxylin and Eosin. The sections obtained were&nbsp; microscopically examined by two pathologist individually.&nbsp; The histological parameters were recorded</p> <p>&nbsp;</p> <p><strong><em>RESULTS AND CONCLUSION: </em></strong></p> <p>Out of the 200 cases, 119 (59.5%) were males and 81 (40.5%) were females with M: F ratio of 1.4:1.The common locations of the tumors as shown in Table no.1 are the right colon (n=131, 65.5%) left colon (n=53, 26.5%), rectum (n=9, 4.5%), synchronous tumors (n=3, 1.5%) and colon with site not specified (n=4,2.0%).</p> <p>Mucinous carcinoma 23 cases, Signet Ring Cell carcinoma 17 cases , Diffuse increase in Intraepithelial Lymphocytes , Undifferentiated Carcinoma 48 cases&nbsp; and Heterogeneous morphologies 35 cases, Tumor Budding in 49 cases ,&nbsp; Crohn's like lymphocytic infiltrate in 3 cases , Synchronous tumor 3 cases , Cribriform morphology in 31&nbsp; cases along with Right sided tumors in 134 cases.</p> .Dr.Priyanka Gupta Priyanka Gupta, Dr Rohan Gupta , Dr.T.P.Sahoo, Dr Abhishek Sharma Copyright (c) 2022 Author (s). Published by Siddharth Health Research and Social Welfare Society https://creativecommons.org/licenses/by/4.0 https://pathology.medresearch.in/index.php/jopm/article/view/583 Thu, 20 Jan 2022 13:05:44 +0530