“The Study of Bacteriological Agents of Chronic Suppurative Otitis Media Aerobic Culture and Antibiotic Sensitivity Testing Pattern at a Tertiary Care Hospital in and around Rajamundry: A cross-sectional study”
Abstract
Introduction: Chronic suppurative otitis media is a chronic infection of the middle ear and mastoid cavity which if not treated leads to partial or total loss of hearing and even life-threatening complications like meningitis and Intracranial abscess. Hence it is important to treat the persistent ear discharge in CSOM according to the antimicrobial sensitivity of the organism isolated.
Methods: This is a prospective cross-sectional study done in 135 clinically diagnosed patients of CSOM at GSL medical college and General Hospital, Rajamundry, Andhra Pradesh from May 2019 to October 2019. Sterile swabs were used to collect pus from discharging ear and were inoculated onto Blood and MacConkey agar for 24-48 hrs, and identification of organisms was done by using standard biochemical reactions and antibiotic sensitivity testing done by using modified Kirby- Bauer method as per CLSI guidelines.
Results: A total of 156 strains were isolated from 135 patients in the present study of which Pseudomonas aeruginosa and Staphylococcus aureus were amongst the most isolated pathogens in culture. Imipenem showed the most sensitivity against Pseudomonas, subsequently levofloxacin and piperacillin. Linezolid was the most sensitive versus Staphylococcus ensued by ciprofloxacin and cloxacillin.
Conclusions: In the present study of Chronic suppurative otitis media patients, Pseudomonas and Staphylococcus were highly prevalent and showed increased resistance to beta-lactams and commonly used antimicrobial drugs. Antimicrobials like imipenem, piperacillin, and quinolones are effective against most cases of chronic suppurative otitis media. Successful treatment and avoidance of antimicrobial resistance would be achieved by the appropriate use of antimicrobials with sufficient evidence.
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Jitendra and Shiv Kumar. Microbiological profile and Antibiogram in cases of Chronic suppurative otitis media at a Tertiary care Hospital, Jaipur. Int.J.Curr.Microbiol.App Sci.2018;7 (01): 395-407. doi: https://doi.org/10.20546/ijcmas.2018.701.045
Klein, Jerome O. Otitis externa, otitis media, and mastoiditis. Mandell, Douglas, and Bennett's principles and practice of infectious diseases (2015): 767.
Dhingra BC, Dhingra S. Diseases of the ear, nose and throat.5thed. New Delhi:Elsevier; 2010.75-83.
Vikas J, Swathi J Bacteriological profile of Chronic suppurative otitis media and Antibiotic Sensitivity Pattern of Aerobic isolates in TCH of Central India. Int J Curr Microbiol App Sci.2019:8(01): 2240-46. DOI:https://doi.org/10.20546/ijcmas.2019.801.234.
Kumar, R., Srivastava, P., Sharma, M., Rishi, S., Nirwan, S., & Hemwaniand, K. Isolation and antimicrobial sensitivity profile of bacterial agents in chronic suppurative otitis media patients at NIMS Hospital. Jaipur. IJPBS, 3.4 (2013): 265-9.
Kumar, S., Sharma, R., Saxena, A., Pandey, A., Gautam, P., & Taneja, V. Bacterial flora of infected unsafe CSOM. Indian journal of Otology, 18.4 (2012): 208.
Naz, R., Farooqui, M. K., Girotra, R., Yadav, M., et al. A. Bacterial profile and antibiotic sensitivity pattern of CSOM patient in Mewat region. J. Evid Based Med. Health, 2.61 (2015): 9051-54.
Sowmya TR, Ravi D, M HanumanthP. Bacteriological Profile of Chronic suppurative otitis media in TCH. Int J Otorhinolaryngology Head Neck Surg 2017;3:601-5.
WHO. State of Hearing and Ear care in SEA region. WHO Regional office for SEA.
Jung TT, Hanson JB. Classification of otitis media and surgical principles. Otolaryngol Clin North Am. 1999 Jun;32(3):369-83. doi: 10.1016/s0030-6665(05)70139-0.
Prakash, M., Lakshmi, K., Anuradha, S., & Swathi, G. N. Bacteriological profile and their antibiotic susceptibility pattern of cases of chronic suppurative otitis media. Asian journal of Pharmaceutical and clinical research, (2013):210-212.
Betty A Fobes, Daniel F Sahm, Alice Weissfields, Bailey & Scott Diagnostic Microbiology, 12th Edition: Elsevier 2007: pp.832-42.
Duguid JP, CollJG, FraserAG. Laboratory Strategy in the diagnosis of infective syndrome In: Mackies & McCartney Practical Medical Microbiology (14 Edition), Churchill Livingstone, London 2004.
Elmer, W. K., et al. A Color Atlas and Text Book of Diagnostic Microbiology. (1997): 122-30.
Clinical & Laboratory Standard Institute, 2012 Performance Standards for Antimicrobial Susceptibility Testing. Clinical & Laboratory Standards Institute, Wayne. 24th Informational supplement.
Ahmed, Ashfaq, Javaid Usman, and Rizwan Hashim. Isolates from chronic suppurative otitis media and their antimicrobial sensitivity. Pak Armed Forces Med J 49.2 (1999): 82-5.
Susmita Kumari Sahu, Moningi Venkata Narasimham, Indrani. Microbiological Profile of Chronic suppurative otitis media & InVitro ASP in a TCH. Otolaryngology Online Journal 2014; 4(4).
Shrestha, B. L., Amatya, R. C. M., Shrestha, I., & Ghosh, I. Microbiological profile of chronic supurative otitis media. Nepalese Journal of ENT Head and Neck Surgery, 2.2 (2011): 6-7.
Ashutosh Rawat, Roma Goyal. A Study of Bacterial Profile and Antibiotic Susceptibility Pattern of CSOM. Intrernational Journal of Microbiolgy Applied Science 2015; 4(8): 23-27.
Akinpelu OV, Amusa YB, Komolafe EO, Adeolu AA, Oladele AO, Ameye SA. Challenges in management of chronic suppurative otitis media in a developing country. J Laryngol Otol. 2008 Jan;122(1):16-20. doi: 10.1017/S0022215107008377.
Hiremath, S. L., Kanta, R. C., Yeshwanathrao, M., & Vasantha Kumar, C. M. Aerobic bacterial isolates of CSOM and their antibiotic sensitivity pattern. The Indian Practitioner, 54.7 (2001): 486-489.
Loy AH, Tan AL, Lu PK. Microbiology of chronic suppurative otitis media in Singapore. Singapore Med J. 2002 Jun;43(6):296-9.
Wadile Rahul Gopichand, Bhate Viraj Madues. Bacteriological Profile of Chronic suppurative otitis media. Int.Journal Current Applied Sciences 2015; 4(6): 41-47.
Swayamsidha Andhale, Parag Swanth, Goel HC. Microbiology of Chronic suppurative otitis media without cholesteatoma. Goa National Journal of Otolaryngology and Head and Neck Surgery, April 2015; 3(12): no.1.
Prakash R, Juyal D, Negi V, Pal S, Adekhandi S, Sharma M, et al. Microbiology of chronic suppurative otitis media in a tertiary care setup of uttarakhand state, India. N Am J Med Sci. 2013 Apr;5(4):282-7. doi: 10.4103/1947-2714.110436.
Maji PK, Chatterjee TK, Chatterjee S, Chakrabarty J, Mukhopadhyay BB. The investigation of bacteriology of chronic suppurative otitis media in patients attending a tertiary care hospital with special emphasis on seasonal variation. Indian J Otolaryngol Head Neck Surg. 2007 Jun;59(2):128-31. doi: 10.1007/s12070-007-0038-x.
Singh, A. H., R. Basu, and A. Venkatesh. Aerobic bacteriology of chronic suppurative otitis media in Rajahmundry, Andhra Pradesh, India. Biology and Medicine 4.2 (2012): 73.
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