Assessment of serological markers of dengue and it’s correlation to thrombocytopenia
Background:Dengue is an acute, potentially fatal viral infection that can culminate in dengue hemorrhagic fever and dengue shock syndrome. The present study was conducted to assess the correlation between platelet counts and serological markers of dengue infection.
Materials and Methods: The present study was conducted among 120 patients suspected of dengue fever. Serum samples were collected from suspected dengue fever patients. The samples were tested for NS1 antigen, IgM, and IgG antibodies using the ICT test kit. The platelet count was recorded in dengue parameter-positive and -negative cases.
Results: Out of 120 patients, males were 70 and females were 50. NS1 only was detected in 62, IgM only in 20, IgG only in 14, NS1 and IgM only in 10, NS1 and IgG only in 8 and IgM and IgG only in 6 cases. 62 patients that were positive for NS 1, thrombocytopenia was seen in 48, 12 out of 20 IgM positive patients, 10 out of 14 IgG positive patients, 6 out of 10 NS1 and IgM only positive patients, 4 out of 8 NS1 and IgG only positive patients and 2 out of 6 IgM and IgG only positive patients were detected. The difference was significant (P< 0.05).
Conclusion:In fever, thrombocytopenia is observed more uniformly in subjects positive for dengue. WheneverIgM is detected as compared to NS1 and can be used asan indicator to reduce the complication of dengue disease. The use of NS1 in the diagnosis of dengue increases the detection rate significantly.
Libraty DH, Young PR, Pickering D, Endy TP, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis. 2002;186(8):1165-1168. doi: https://doi.org/10.1086/343813.
Malavige GN, Gomes L, Alles L, Chang T, Salimi M, Fernando S, et al. Serum IL-10 as a marker of severe dengue infection. BMC Infect Dis. 2013;13(1):341. doi: https://doi.org/10.1186/1471-2334-13-341.
WHO: Dengue guidelines for diagnosis, prevention and control. In Southeast Asian Office of the WHO: World Health Organisation; 2009.
Jayaratne S, Atukorale V, Gomes L, Chang T, Wijesinghe T, Fernando S, et al. Evaluation of the WHO revised criteria for classification of clinical disease severity in acute adult dengue infection. BMC Res Notes. 2012;5:645. doi: https://doi.org/10.1186/1756-0500-5-645.
Gupta V, Yadav TP, Pandey RM, Singh A, Gupta M, Kanaujiya P, et al. Risk factors of dengue shock syndrome in children. J Trop Pediatr. 2011;57(6):451-456. doi: https://doi.org/10.1093/tropej/fmr020.
Malavige GN, Huang LC, Salimi M, Gomes L, Jayaratne SD, Ogg GS. Cellular and cytokine correlates of severe dengue infection. PLoS One. 2012;7(11):50387. doi: https://doi.org/10.1371/journal.pone.0050387.
Lee LK, Gan VC, Lee VJ, Tan AS, Leo YS, Lye DC. Clinical relevance and discriminatory value of elevated liver aminotransferase levels for dengue severity. PLoSNegl Trop Dis. 2012;6(6):1676. doi: https://doi.org/10.1371/journal.pntd.0001676.
Tricou V, Vu HT, Quynh NV, Nguyen CV, Tran HT, Farrar J, et al. Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infect Dis. 2010;10:142.doi: https://doi.org/10.1186/1471-2334-10-142.
Guzmán MG, Kourí G. Dengue: An update. Lancet Infect Dis. 2001;2:33-42.doi: https://doi.org/10.1016/s1473-3099(01)00171-2.
Paranavitane SA, Gomes L, Kamaladasa A, Adikari TN, Wickramasinghe N, Jeewandara C, et al. Dengue NS1 antigen as a marker of severe clinical disease. BMC Infect dis. 2014;14(1):570.doi: https://doi.org/10.1186/s12879-014-0570-8.
Jyothi P, Metri BC. Correlation of serological markers and platelet count in the diagnosis of Dengue virus infection. AdvanBiomed Res. 2015;4:26.doi: https://doi.org/10.4103/2277-9175.150396.
Kulkarni RD, Patil SS, Ajantha GS, Upadhya AK, Kalabhavi AS, Shubhada RM, et al. Association of platelet count and serological markers of dengue infection- importance of NS1 antigen. Indian J Med Microbiol. 2011;29:359-62.doi: https://doi.org/10.4103/0255-0857.90159.
Copyright (c) 2020 Author (s). Published by Siddharth Health Research and Social Welfare Society
This work is licensed under a Creative Commons Attribution 4.0 International License.