Speciation of Candida using CHROMagar isolated from Various Clinical Samples

  • Dr. Shwetha D C Assistant Professor, Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B G Nagarac
  • Dr. Venkatesha D. Professor and Head, Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B G Nagara, Karnataka, India
Keywords: Candida albicans, Hicrome agar, Non albicans Candida

Abstract

Introduction: Infections due to Candida especially Non-albicans Candida (NAC) species are increasingly being reported in recent years. Isolation and identification up to species level are of utmost importance in the early management of these infections.

Materials and methods: All the clinical samples received in the Microbiology laboratory were inoculated on to Blood agar and MacConkey agar. The isolates which revealed Gram-positive budding yeast cells on Gram staining were further inoculated on to SDA (Sabouraud Dextrose agar) and Hicrome Candida differential agar. Candida was differentiated into species based on color and morphology on Hicrome agar.

Result: A total of 126 Candida were isolated from various clinical samples. C.Albicans was the most common species isolated (49.2%) followed by C. tropicalis (19.8%), C. glabrata (19%), and C. krusei (12%).

Conclusion: Hicrome agar is a simple, rapid, and cost-effective medium for the speciation of Candida.

Downloads

Download data is not yet available.

References

Deorukhkar SC, Saini S, Mathew S.Non-albicans Candida infection: An Emerging Threat. Interdisciplinary Perspectives on Infectious Diseases. 2014. doi: http://dx.doi.org/10.1155/2014/615958.

Sardi JCO, Scorzoni L, Bernardi T, et al.Candidaspecies:current epidemiology, pathogenicity, Biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol.2013;62(1):10-24.doi: http://dx.doi.org/10.1099/jmm.0.045054-0.

Vijaya D, Harsha TR, Nagaratnamma T. Candida Speciation Using Chom Agar. J Clin Diagnos Res. 2011;5(4):755-757.

Manjunath V, GS Vidya, Sharma A, Prakash MR, Murugesh. Speciation of Candida by HiCrome agar and Sugar assimilation test in both HIV infected and non-infected patients. Int J Biol Med Res. 2012;3(2):1778-1782.

Jayachandran AL, Katragadda R, Ravinder T, Vajravelu L, Manoranjan L, Hemalatha S. Antifungal Susceptibility Pattern among Candida species: An Evaluation of Disc Diffusion and Broth Micro-dilution Method. JMicrobiol Infect Dis.2018;8(3):97-101. doi: https://doi.org/10.5799/jmid.458457.

Shettar SK, Patil AB, Nadagir SD, Shepur TA, Mythri BA, Gadadavar S. Evaluation of HiCrome differential agar for speciation of Candida. JAcad Med Sci. 2012;2(3):102-104.doi: https://doi.org/10.4103/2249-4855.132950.

Forbes BA, Sahm DF, Weissfield AS. Laboratory methods in basic Mycology. Bailey and Scott’s diagnostic Microbiology 12thedition, USA:Mosby Elsevier 2007:629-716.

Sankari SL, Mahalakshmi K, Kumar VN. Chromogenic medium versus PCR-RFLP in the speciation of Candida: A comparative study. BMC Research Notes 2019;12:681. doi: https://doi.org/10.1186/s13104-019-4710-5.

Amit Bhosale, S J Ghosh, P G Shadija. Identification of various Candida species by using CHROMagar Candida: A rapid screening method. Int J Rec Trends Sci Technol. 2015;16(3):596-598.

Soumya Kaup, Jaya Sankarankutty, H VBalasubramanya, Suma Kulkarni, M Nirmala. Speciation of Candida using HiChrome Candida Differential Agar. Int J Curr Microbiol App Sci. 2016;5(7):267-274. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.027.

Samyuktha AA, Saikumar C. Isolation, Identification and Speciation of Candida Species from Various Clinical Specimens in a Tertiary Care Hospital in Chennai. Scholar J App Med Sci. 2017;5(8F):3460-3468.doi: http://dx.doi.org/10.21276/sjams

Madhumathi B, Rajendran R. Evaluation of Chrome Agar in Speciation of Candida Species from Various Clinical Samples in a Tertiary Care Hospital. Int JCurr Microbiol App Sci. 2015;4(9):463-472.

Marak MB, Dhanashree B. Antifungal Susceptibility and Biofilm Production of Candida Spp. Isolated from Clinical Samples. Int J Microbiol. 2018. doi: https://doi.org/10.1155/2018/7495218.

Dharmeshwari T, Chandrakesan SD, Mudhigeti N, Perticia A, Kanungo R. Use of Chromogenic Medium for Speciation of Candida isolated from Clinical Specimens. Int J Cur Res Rev. 2014;6(1):1-5.

Deorukhkar SC, Roushani S. Identification of Candida Species: Conventional Methods in the Era of Molecular Diagnosis. Ann Microbiol Immunol. 2018;1(1):1002.

Kumar S, Vyas A, Kumar M, Mehra SK. Application of CHROMagar Candida for Identification of Clinically Important Candida species and their Antifungal Susceptibility Pattern. Int J Biol Med Res. 2013;4(4):3600-3606.

Jangla SM, Naidu R, Patel SC. Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai. Int J Biomed Res. 2018;9(3):106-111. doi: https://doi.org/10.7439/ijbr.v9i3.4678.

Rao RP. Isolation, identification and speciation of candida species from various clinical samples in a tertiary care teaching hospital in Karnataka, India. J Evid Based Med Health. 2019;6(11):866-868.doi: https://doi.org/10.18410/jebmh/2019/182.

B VigneshKanna, G Amar Kumar, M Swapna, Easow JM. Isolation and identification of Candida species from various clinical samples in a tertiary care hospital. Int J Res Med Sci. 2017;5(8):3520-3522. doi: http://dx.doi.org/10.18203/2320-6012.ijrms20173554.

Das KH, Getso M. Distribution of Candida albicans and non-albicans Candida in clinical samples and their intrinsic biofilm production status. Int J Med Sci Public Health. 2016;5(12):2443-2447. doi: https://doi.org/10.5455/ijmsph.2016.26042016491.

Saxena N, Maheshwari D, Dadhich D, Singh S. Evaluation of Congo Red Agar for Detection of Biofilm Production by Various Clinical Candida Isolates. JEvol Med Dent Sci. 2014;3(59):13234-13238.doi: https://doi.org/10.14260/jemds/2014/3761.

Rudrappa PT, Chandrashekar SC, Sumana MN. Speciation of Candida Isolates from Clinical Samples by Using Conventional and Chrom agar method. Int J Clin Microbiol App Sci. 2018;7(3):2663-2668. doi: https://doi.org/10.20546/ijcmas.2018.703.307.

Kaur R, Dhakad MS, Goyal R, Kumar R. Emergence of non-albicans Candida species and antifungal resistance in intensive care unit patients. Asian Pacific J Trop Biomed. 2016;6(5):455-460. doi: http://dx.doi.org/10.1016/j.apjtb.2015.12.019.

Published
2020-04-30
How to Cite
DC, S., & D., V. (2020). Speciation of Candida using CHROMagar isolated from Various Clinical Samples. Pathology Update: Tropical Journal of Pathology and Microbiology, 6(4), 303-308. https://doi.org/10.17511/jopm.2020.i04.06
Section
Original Article