Association of biofilm production in ESBL and MBL producing clinical isolates of Pseudomonas aeruginosa

  • Dr. Kulkarni D.M. Associate Professor, Department of Microbiology, S.R.T.R Government Medical College, Ambajogai, Maharashtra, India
  • Dr. Nilekar S.L. Professor and HOD, Department of Microbiology, S.R.T.R Government Medical College, Ambajogai, Maharashtra, India
  • Dr. Vidhya Thirumalaisamy Resident, S.R.T.R Govt Medical college
Keywords: Antibiogram, Biofilm, ESBL, MBL, Pseudomonas aeruginosa

Abstract

Introduction: Pseudomonas aeruginosa is one of the most prevalent nosocomial pathogens that cause a life-threatening infection. One of the important characteristics of P. aeruginosa is biofilm formation and the most studied bacterium related to biofilm formation so far. The biofilm formation and beta-lactamases production synergistically contribute to the extensive dissemination of multi-drug resistant strains.

Aim: The present study was conducted to identify, biofilm-producing isolates of P. aeruginosa along with their antibiotic resistance pattern and ESBL and MBL production and to analyze their antibiogram.

Materials and methods: Various clinical specimens were collected and totally 82 clinical isolates of P. aeruginosa were included in this study. Biofilm producing isolates were identified by the tube adherence method.

Results: Among the total, 22 [26.83%] isolates were biofilm producers and the maximum number was obtained from blood [100%], followed by ETT [75%], and Drain [66.67%]. Biofilm producing isolates were showing more resistance in comparison to non-biofilm producers. All isolates of P. aeruginosa were sensitive to colistin and polymyxin B. Association of ESBL production and biofilm formation found to be statistically significant [p < 0.002], which was a contrast to association of MBL production and biofilm formation.

Conclusion: High-level resistance to antimicrobial agents is a characteristic feature of infection caused by biofilm and lead to chronic infections. Knowledge about these biofilm-producing isolates is important in the clinical setting to eradicate these chronic and life-threating infections.

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Published
2020-02-29
How to Cite
Kulkarni, D., Nilekar, S., & Thirumalaisamy, V. (2020). Association of biofilm production in ESBL and MBL producing clinical isolates of Pseudomonas aeruginosa. Pathology Update: Tropical Journal of Pathology and Microbiology, 6(2), 175-181. https://doi.org/10.17511/jopm.2020.i02.10
Section
Original Article