Prevalence of extended spectrum B lactamase producing E.coli and Klebsiella SPP isolated in a tertiary care hospital, Gujarat
Keywords:
Bhuj, E.coli, extended spectrum B lactamase, Gram negative bacilli
Abstract
Background: Antibiotic resistance among gram negative bacilli is a rapidly expanding problem due to the organism’s ability to mutate and to acquire the transmit plasmid and other genetic elements encoding resistance genes.
Objective: This study was conducted to know the prevalence of ESBLs in E. coli and Klebsiella spp. isolates obtained from clinical samples. Material and Methods: A detailed history was taken and Performa was filled for each patient documenting age, sex, history of illness was obtained. Study was conducted at microbiology Department, Gujarat Adani Institute of Medial Sciences, Bhuj from May 2014 to Dec 2015. Total 2500 clinical samples like Urine, Sputum, Blood, Pus, CSF, Pleural fluid were collected in sterile containers. Samples from which E.coli and Klebsiella spp were isolated were considered for this study. Detection of ESBL was done as per CLSI guidelines.
Results: Total 2500 clinical samples were tested for culture and sensitivity during August 2015 to July 2017. Out of this 500 samples showed growth of E. coli and Klebsiella spp. Among 500 isolates coli had 268 and from them 189 were ESBL positive. Similarly 232 were isolates from Klebsiella spp and among them 185 were positives for ESBL.
Conclusion: In the present study, ESBL prevalence was 49.99% (E. coli = 50.53% and K. pneumoniae = 49.46%). A moderately high prevalence of ESBL producing E. coli and K. pneumoniae was observed and confirmed in the urine, sputum, pus, CSF and Blood. A strict hospital infection control policy and a prudent anti-microbials use regimens are to be adopted by the physicians.
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References
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22. Goossens H; MYSTIC Study Group (Europe). MYSTIC program: summary of European data from 1997 to 2000. Diagn Microbiol Infect Dis. 2001;41 (4): 183-9. DOI: https://doi.org/10.1016/ S0732-8893(01) 00320-0
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24. Babypadmini S, Appalaraju B. Extended spectrum -lactamases in urinary isolates of Escherichia coli and Klebsiella pneumoniae - prevalence and susceptibility pattern in a tertiary care hospital. Indian J Med Microbiol. 2004;22(3):172–4.
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2. Ali AM, Rafi S, Qureshi AH. Frequency of Extended Spectrum Beta Lactamase Producing Gram Negative Bacilli among Clinical Isolates at Clinical Laboratories of Army Medical College, Rawalpindi. http://ayubmed. edu.pk/ JAMC/PAST/16-1/Aarif.htm
3. Yazdi M, Nazemi A, Mirinargasi M, Jafarpour M, Sharifi SH. Genotypic versus Phenotypic methods to detect extended-spectrum beta-lactamases (ESBLs) in uropathogenic Escherichia coli.Ann Biol Res 2012; 3: 2454-8.
4. Gupta S, Maheshwari V. Prevalence of ESBLs among Enterobacteriaceae and their antibiotic resistance pattern from various clinical samples. Int J Curr Microbiol App Sci 2017;6(9):2620-8. DOI: https://doi. org/ 10.20546/ijcmas. 2017.609.323
5. Clinical laboratory standards institute. Performance standard for antimicrobial susceptibility testing; seventeenth informational supplement M100-S17, vol 27 No.1. Clinical laboratory standards institute. Wayne PA. USA 2007.
6. Mohamudha Parveen R, Harish BN, Parija SC. Ampc Beta lactamases among gram negative clinical isolates from a tertiary hospital, South India. Braz J Microbiol. 2010;41(3):596-602. DOI:10.1590/S1517-8382201 00 00300009. Epub 2010 Sep 1.
7. Shashwati N, Kiran T, Dhanvijay AG. Study of extended spectrum β-lactamase producing Enterobacte-riaceae and antibiotic coresistance in a tertiary care teaching hospital. J Nat Sci Biol Med. 2014;5(1):30–35. DOI:10.4103/0976-9668.127280.
8. Mathur P, Kapil A, Das B, Dhawan B. Prevalence of extended spectrum beta lactamase producing gram negative bacteria in a tertiary care hospital. Indian J Med Res. 2002;115:153-7.
9. Grover N, Sahni AK, Bhattacharya S. Therapeutic challenges of ESBLS and AmpC beta-lactamase producers in a tertiary care center. Med J Armed Forces India. 2013;69(1):4-10. DOI: 10.1016/j.mjafi. 2012.02. 001. Epub 2012 Jul 17.
10. Hansotia JB, Agarwal V, Pathak AA, Saoji AM. Extended spectrum beta-lactamase mediated resistance to third generation cephalosporins in Klebsiella pneumoniae in Nagpur, central India. Indian J Med Res. 1997;105:158-61.
11. Lal P, Kapil A, Das BK, Sood S. Occurrence of TEM & SHV gene in extended spectrum beta-lactamases (ESBLs) producing Klebsiella sp. isolated from a tertiary care hospital. Indian J Med Res. 2007; 125(2):173-8.
12. Rodrigues C, Joshi P, Jani SH, Alphonse M, Radhakrishnan R, Mehta A. Detection of β-lactamases in nosocomial gram negative clinical isolates. Indian J Med Microbiol. 2004;22(4):247–50.
13. Ananthan S, Subha A. Cefoxitin resistance mediated by loss of a porin in clinical strains of Klebsiella pneumoniae and Escherichia coli. Indian J Med Microbiol. 2005;23(1):20-3.
14. Mendelson G, Hait V, Ben-Israel J, Gronich D, Granot E, Raz R. Prevalence and risk factors of extended-spectrum beta-lactamase-producing Esche-richia coli and Klebsiella pneumoniae in an Israeli long-term care facility. Eur J Clin Microbiol Infect Dis. 2005;24(1):17-22. DOI:10.1007/s10096-004-1264-8
15. Bazzaz BS, Naderinasab M, Mohamadpoor AH, Farshadzadeh Z, Ahmadi S, Yousefi F. The prevalence of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae among clinical isolates from a general hospital in Iran. Acta Microbiol Immunol Hung. 2009;56(1):89-99. DOI: 10.1556/ AMicr. 56.2009.1.7.
16. Saurina G, Quale JM, Manikal VM, Oydna E, Landman D. Antimicrobial resistance in Enterobacte-riaceae in Brooklyn, NY: epidemiology and relation to antibiotic usage patterns. J Antimicrob Chemother. 2000; 45 (6): 895-8. DOI:10.1093/jac/45.6.895
17. Romero ED, Padilla TP, Hernández AH, Grande RP, Vázquez MF, García IG. Prevalence of clinical isolates of Escherichia coli and Klebsiella spp. producing multiple extended-spectrum beta-lactamases. Diagn Microbiol Infect Dis. 2007;59(4):433-7. Epub 2007 Oct 29. DOI:10.1016/j.diagmicrobio.2007.06.007
18. Kuo KC, Shen YH, Hwang KP. Clinical implications and risk factors of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae infection in children: a case-control retrospective study in a medical center in southern Taiwan. J Microbiol Immunol Infect. 2007;40(3):248-54.
19. Hoşoğlu S, Gündes S, Kolayli F, Karadenizli A, Demirdağ K, Günaydin M et al. Extended-spectrum beta-lactamases in ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates in Turkish hospitals. Indian J Med Microbiol. 2007;25(4):346-50. DOI: 10.4103/0255-0857.37336
20. Messai Y, Iabadene H, Benhassine T, Alouache S, Tazir M, Gautier V, et al. Prevalence and characteri-zation of extended-spectrum beta-lactamases in Klebsiella pneumoniae in Algiers hospitals (Algeria). Pathol Biol (Paris). 2008;56(5):319-25. DOI: 10.1016/j. patbio. 2008.05.008. Epub 2008 Jun 27.
21. Xiong Z, Zhu D, Zhang Y, Wang F. [Extended-spectrum beta-lactamase in Klebsiella pneumoniae and Escherichia coli isolates]. Zhonghua Yi Xue Za Zhi. 2002; 82(21):1476-9.
22. Goossens H; MYSTIC Study Group (Europe). MYSTIC program: summary of European data from 1997 to 2000. Diagn Microbiol Infect Dis. 2001;41 (4): 183-9. DOI: https://doi.org/10.1016/ S0732-8893(01) 00320-0
23. Duttaroy B, Mehta S. Extended spectrum b lactamases (ESBL) in clinical isolates of Klebsiella pneumoniae and Escherichia coli. Indian J Pathol Microbiol. 2005;48(1):45-8.
24. Babypadmini S, Appalaraju B. Extended spectrum -lactamases in urinary isolates of Escherichia coli and Klebsiella pneumoniae - prevalence and susceptibility pattern in a tertiary care hospital. Indian J Med Microbiol. 2004;22(3):172–4.
25. Babypadmini S, Appalaraju B. Extended spectrum -lactamases in urinary isolates of Escherichia coli and Klebsiella pneumoniae - prevalence and susceptibility pattern in a tertiary care hospital. Indian J Med Microbiol. 2004;22(3):172-4.
CITATION
DOI: 10.17511/jopm.2019.i06.11
Published: 2019-06-30
How to Cite
Dr. Hitesh Assudani, & Dr. Jigar Gusani. (2019). Prevalence of extended spectrum B lactamase producing E.coli and Klebsiella SPP isolated in a tertiary care hospital, Gujarat. Tropical Journal of Pathology and Microbiology, 5(6), 403-407. https://doi.org/10.17511/jopm.2019.i06.11
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