Comparative study of expression of keratins 8, 10, 13 and 17 in CIN III and invasive carcinoma of cervix
Abstract
Introduction: The role of keratin expression patterns as candidate tumour markers continues to be under investigation in human cervix carcinogenesis. Keratin comprise of family of at least 20 intermediate filament proteins that have a specific distribution pattern in epithelial tissue.
Objective: The present study was conducted with an aim to identify CINI, II and CINIII in tissue sections with the help of immunohistochemistry of specific diagnostic markers so as to reduce the burden of invasive cervical carcinoma and to evaluate the role of cytokeratin 8,10,13 and 17 for differentiating CINIII from cervical carcinoma along with its correlation with histopathological diagnosis of these lesions.
Method: We examined the immunohistochemical staining of CK8, CK10, CK13 and CK17 in 64 cases of reference cervix, CINIII lesions and invasive cervical carcinoma.
Results: In present study cytokeratin 8 has sensitivity 40% and specificity 100%, cytokeratin 10 has sensitivity 80% and specificity 40%, cytokeratin 13 has sensitivity 100% and specificity 80% and cytokeratin 17 has sensitivity 40% and specificity 100% in invasive cervical carcinoma. In the CIN III lesions, cytokeratin 8 has sensitivity 56% and specificity 100%, cytokeratin 10 has sensitivity 80% and specificity 79%, cytokeratin 13 has sensitivity 100% and specificity 75% and cytokeratin 17 has sensitivity 72% and specificity 100% in cervical intraepithelial lesion III.
Conclusions: We observed that expression of keratins 8 and 17 and loss of keratins 10 and 13 are good markers of malignant transformation in human cervix. Keratin expression patterns, namely expressions of keratin 10 can be useful for studying and grading squamous cell carcinomas of the cervix.
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References
2. Kent A. HPV Vaccination and Testing. Rev Obstet Gynecol. 2010 Winter;3(1):33-4.[pubmed]
3. Morrison AS.Screening in chronic disease.2nd ed.Newyork: Oxford University Press; 1992.Introduction ;pp 3-42.
4. GlOBOCON 2002, IARC 2009" database: summary table by cancer". Archived from the original on 2008-06-16. Retrieved 2008-10-26.
5. FDA Licenses New Vaccine for Prevention of Cervical Cancer". U.S. Food and Drug Administration. 2006-06-08.[pubmed]
6. W. Frank, E Schmid, K. Weber and M. Osborn.(June1979).Intermediate -sized filaments of human endothelial cells” The journal of cell biology 1979 june;81(3):570-80.doi: 10.1083/jcb.81.3.570.
7. Smedts F, Ramaekers F, Troyanovsky S, et al. Keratin expression in cervical cancer. Am J Pathol. 1992 Aug;141(2):497-511.[pubmed]
8. Maddox P, Sasieni P, Szarewski A, et al. Differential expression of keratins 10, 17, and 19 in normal cervical epithelium, cervical intraepithelial neoplasia, and cervical carcinoma. J Clin Pathol. 1999 Jan;52(1):41-6.[pubmed]
9. Nartam Sharma, Veena Sharma, Prem Raj Singh, et al. Biomed Res-India 2012; 23(4):547-550.
10. Nazir Ahmed, Razia Mehboob. Cervical intraepithelial- neoplasia. Recent trends in diagnosis & treatment. Pakistan J Med Research, 2002;Apr-Jun ;41(2): 43-5.
11. Torrisi A, Castagnali B, Minucci D, ACOG Committee on gynaecology practice, ACOG , Practice Bulletin no.109;cervical cytology screening, Obstetrics Gyneacol, 2009 Dec; 114(6): 1409-1420.doi10.1097/AOG.0b013e3181c6f8a4.
12. Herbart A, Smith JA, Department of Histopathology, Southampton General Hospital, Southampton University Hospitals NHS trust, UK Cytopathology,1999 June;10(3): 161-70.
13. Jha, A., Jha, J., Bista, R., Basnet, B., Kandel, P., Lama, G., Banthia, P., &Thakali, K. A Scenario of Cervical Carcinoma in a Cancer Hospital. Journal of Nepal Medical Association, 2009;48(175). https://doi.org/10.31729/jnma.180.
14. Schiffman MH, Brinton LA. The epidemiology of cervical carcinogenesis. Cancer. 1995 Nov 15;76(10 Suppl):1888-901.[pubmed].[pubmed]
15..Park TW, Fujiwara H, Wright TC. Molecular biology of cervical cancer and it’s precursors.Cancer 1995 nov;76(10 suppl):1902-1913.
16. ZoeR.Edelstein, Margaret M, Madeleine, James PHughes. Cancer Epidemiol Biomarkers Prev 2009;18(4): 1070-1076. doi: 10.1158/1055-9965.EPI-08-0707.
17. Parveen S, Hakim S, Siddiqui S, Ahmed J. A retrospective study of female genital tract malignancies. Jour of Med Sc and Tech; 2009;Sep 12;1(3): 40-43.
18. Satya B. Paul, Basant K,Tiwari, Arun Paul Choudhary, Department of Chemistry, Assam Uni, Silichar, Assam, Assam Uni, Journal of sci and Tech, Biological and environmental science,.2011; 7(1):36-42
19. Goellner J.R.et al.Carcinoma of cervix, clinicipathologicalcoorelation Am. J. Clinicol ,Pathol,1976; 66( 5) : 775-786.
20. Verma K, Kapila K. Increase in accuracy of typing of carcinoma of the uterine cervix by combined use of histology and cytology. Acta Cytol. 1982 Mar-Apr;26(2):131-4.
21. Subir M, Gangare N, Bnake A, Ingote N, et al. Carcinoma of the cervix and stain for mucin. Journal of Obs and gynae of India. 1991;1(5): 678-680.
22. Carla Carriho, Matos Alberto.Keratins 8, 10, 13, and 17 are useful markers in the diagnosis of human cervix carcinomas. Human pathology 2004 May; 35(5); 546- 51. https//doi.org/10.1016/j.humanpath.2004.01.02.
23. Ikeda K, Tate G, Suzuki T, et al. Coordinate expression of cytokeratin 8 and cytokeratin 17 immunohistochemical staining in cervical intraepithelial neoplasia and cervical squamous cell carcinoma: an immunohistochemical analysis and review of the literature. Gynecol Oncol. 2008 Mar;108(3):598-602. doi: 10.1016/j.ygyno.2007.11.042. Epub 2008 Jan 14.
24. SmaroulaDivani, George Kalodimos; Archive of oncology, 2010 october; 18(3): 88-90.doi 10.2298/AOO1003088D
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