Prevalence of MBL producing pseudomonas aeruginosa in various clinical specimens in tertiary care hospital, Karimnagar
Keywords:
Pseudomonas aeruginosa, Combined Disc Test, Imipenem-EDTA double disc synergy test, metallo-β- lactamases, Imipenem (IPM)
Abstract
Aim: To find the prevalence of MBL Producing Pseudomonas aeruginosa from various clinical specimen.
Material and Methods: A total 114 cases from which Pseudomonas aeruginosa has been isolated from Swab, Urine, pus, Sputum, Bal, Foley’s catheter, E.T. Secretion received from various clinical departments. The study was carried outovera period of Six months. The isolates were tested by IPM-EDTA Combined Disc Test (CDT) and Imipenem-EDTA double disc synergy test (DDST). Descriptive statistics and chi-square is used with the help of MS Excel and SPSS Version 25
Results: Among 114 Pseudomonas aeruginosa isolated from various clinical specimens, 16 (14.03%) imipenem-resistant Pseudomonas isolates. Out of 16 IPM resistant, 15(93.75%) were positive for MBL by CDT-IPM method and 11 (68.75%) were positive by Imipenem-EDTA (DDST) method, respectively.
Conclusion: Increase in the resistant pattern of antibiotics can lead to increased morbidity, mortality and economic burden on patients. So it is necessary to detect MBL producing Pseudomonasaeruginosa by simple and effective methods.
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References
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2. Kali A, Srirangaraj S, Kumar S, et al. Detection of metallo-beta-lactamase producing Pseudomonas aeruginosa in intensive care units. Australas Med J. 2013 Dec 31;6(12):686-93. doi: 10.4066/AMJ.2013.1824. eCollection 2013.[pubmed]
3. A. C. Rodloff, E. J. C. Goldstein, A. Torres, Two decades of imipenem therapy. Journal of Antimicrobial Chemotherapy. Volume 58, Issue 5, November 2006, Pages 916–929,
4. Egorov AM, Ulyashova MM, Rubtsova MY. Bacterial Enzymes and Antibiotic Resistance. Acta Naturae. 2018 Oct-Dec;10(4):33-48.[pubmed]
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7. Peleg AY, Franklin C, Bell JM, et al. Dissemination of the metallo-beta-lactamase gene blaIMP-4 among gram-negative pathogens in a clinical setting in Australia. Clin Infect Dis. 2005 Dec 1;41(11):1549-56. Epub 2005 Oct 31.[pubmed]
8. Koneman's Color Atlas and textbook of Diagnostic Microbiology. 5th ed. USA: Lippincott Williams and Wilkins Company; 2005.
9. Clinical Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Twentieth informational supplement. CLSI document M100-S20. Wayne, PA: CLSI; 2010.
10. Yong D, Lee K, Yum JH, et al. Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol. 2002 Oct;40(10):3798-801.[pubmed]
11. Lee K, Lim YS, Yong D, et al. Evaluation of the Hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-beta-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol. 2003 Oct;41(10):4623-9.[pubmed]
12. Sood S. Phenotypic Tests for detecting incidence of Metallo-Β-Lactamase (Mbl) Producing Pseudomonas aeruginosa in Jaipur. National J Laboratory Medi. 2014;3(2):22-7.
13. Mehta A, Tukaram P. Detection and characterization of metallo-β-lactamases producing Pseudomonas aeruginosa clinical isolates at a tertiary care hospital of Bhopal, India. Int J Res Med Sci 2016; 4:4084-8.
14. Navaneeth BV, Sridaran D, Sahay D, et al. A preliminary study on metallo-beta-lactamase producing Pseudomonas aeruginosa in hospitalized patients. Indian J Med Res. 2002 Dec;116:264-7.[pubmed]
15. Watanabe M, Iyobe S, Inoue M, et al. Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1991 Jan;35(1):147-51.[pubmed]
16. Yong D, Lee K, Yum JH, et al. Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol. 2002 Oct;40(10):3798-801.[pubmed]
17. Soumya S. and Mahantesh B. Nagmoti.. Comparison of Different Phenotypic Tests for the Detection of Metallo-B-Lactamase (MBL) Producing Pseudomonas aeruginosa. Int. J. Curr. Microbiol. App.Sci. 2017; 6(10): 3231-3238.
18. Sujatha, R., Roma Goyal and Vikas Mishra. Detection of Metallo Beta Lactamase Producing Pseudomonas aeruginosa among Clinical Isolates. Int. J. Curr. Microbiol. App. Sci. 2017; 6(2): 1567-1573.
19. Irfan S, Zafar A, Guhar D, et al. Metallo-beta-lactamase-producing clinical isolates of Acinetobacter species and Pseudomonas aeruginosa from intensive care unit patients of a tertiary care hospital. Indian J Med Microbiol. 2008 Jul-Sep;26(3):243-5.[pubmeed]
20. Attal RO, Basak S, Mallick SK, Bose S. Metallo-beta-lactamase producing Pseudomonas aeruginosa: An emerging threat to clinicians. J Clin Diagn Res. 2010; 4:2691-6.
21. Fam N, Diab M, Helmi H, El-Defrawy I. Phenotypic detection of metalloβ-lactamases and extended spectrumβ-lactamases among gram negative bacterial clinical isolates. Egypt J Med Microbiol. 2006; 15:719-30.
22. Jesudason MV, Kandathil AJ, Balaji V. et al. Comparison of two methods to detect carbapenemase & metallo-beta- lactamase production in clinical isolates. Indian J Med Res. 2005 Jun;121(6):780-3.[pubmed]
23. John S, Balagurunathan R. Metallo beta lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii. Indian J Med Microbiol. 2011 Jul-Sep;29(3):302-4. doi: 10.4103/0255-0857.83918.[pubmed]
24. Ghasemian A, Salimian Rizi K, Rajabi Vardanjani H, et al. Prevalence of Clinically Isolated Metallo-beta-lactamase-producing Pseudomonas aeruginosa, Coding Genes, and Possible Risk Factors in Iran. Iran J Pathol. 2018 Winter;13(1):1-9.[pubmed]
25. Franklin C, Liolios L, Peleg AY. Phenotypic detection of carbapenem-susceptible metallo-beta-lactamase-producing gram-negative bacilli in the clinical laboratory. J Clin Microbiol. 2006 Sep;44(9):3139-44. DOI:10.1128/JCM.00879-06.[pubmed]
CITATION
DOI: 10.17511/jopm.2019.i04.04
Published: 2019-04-30
How to Cite
Dr. Amar C. Sajjan, Mr. Sachin R.Gurnule, & Dr. B. Aparna. (2019). Prevalence of MBL producing pseudomonas aeruginosa in various clinical specimens in tertiary care hospital, Karimnagar. Tropical Journal of Pathology and Microbiology, 5(4), 205-209. https://doi.org/10.17511/jopm.2019.i04.04
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