Correlation of serological markers with haematological parameters in early diagnosis of dengue infection in dengue prone areas
Abstract
Background: Dengue is a serious, rapidly spreading tropical mosquito-borne viral disease caused by dengue virus belonging to the family flaviviridae and genus flavivirus in the last five decades. In many dengue endemic remote areas with limited laboratory diagnostic services their rapid diagnostic tests are simple method which provides the immediate diagnosis and early management.
Materials and methods: The study includes clinically suspected 1236 cases with all haematological parameters and tested at clinical laboratory in which 500 serum samples showed positivity for one or more markers like NS1, IgM or IgG antibodies.
Results: In our study among 500 seropositive cases thrombocytopenia (<1 lakh) was evident in 66.7% and in 736 seronegative cases thrombocytopenia was seen in 27.47%. Out of 323 NS1 positive cases, thrombocytopenia was evident in 56.0% and in exclusive antibody positive cases thrombocytopenia was noted in 19.2%. These associations were considered to be statistically significant.
Conclusion: The problem of dengue is complex in India because large population, poor medical and diagnostic facilities and inadequate mosquito control. Therefore, studies like this will significantly contribute to the early diagnosis, management and can reduce patient’s morbidity and mortality.
Downloads
References
2. Monath TP. Dengue: the risk to developed and developing countries. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2395-400.
3. Gubler DJ. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev. 1998 Jul;11(3):480-96. [PubMed]
4. Leitmeyer KC, Vaughn DW, Watts DM, Salas R, Villalobos I, de Chacon, Ramos C, Rico-Hesse R. Dengue virus structural differences that correlate with pathogenesis. J Virol. 1999 Jun;73(6):4738-47. [PubMed]
5. Lanciotti RS, Lewis JG, Gubler DJ, Trent DW. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol. 1994 Jan;75 ( Pt 1):65-75. [PubMed]
6. Messer WB, Gubler DJ, Harris E, Sivananthan K, de Silva AM. Emergence and global spread of a dengue serotype 3, subtype III virus. Emerging Infectious Diseases, 2003, 9(7):800--809. http://www.cdc.gov/ncidod/EID/vol9no7/03-0038.htm
7. Rodenhuis-Zybert IA, Wilschut J, Smit JM. Dengue virus life cycle: viral and host factors modulating infectivity. Cell Mol Life Sci. 2010 Aug;67(16):2773-86. doi: 10.1007/s00018-010-0357-z. Epub 2010 Apr 6. [PubMed]
8. Normile D. Tropical medicine. Surprising new dengue virus throws a spanner in disease control efforts. Science. 2013 Oct 25;342(6157):415. doi: 10.1126/science.342.6157.415.
9. Kaihatsu K, Harada E, Matsumura H, Takenaka A, Wichukchinda N, et al., (2016) Future Perspective of Nucleic Acid-based Detection of Dengue Virus and its Serotypes. J Antivir Antiretrovir 8:LXIX-LXXII. doi:10.4172/jaa.1000e132.
10. Magpusao NS, Monteclar A, Deen JL.Slow improvement of clinically diagnosed dengue haemorrhagic fever case fatality rates. Trop Doct, 200:33(3):156-159.
11. Magpusao NS, Monteclar A, Deen JL. Slow improvement of clinically-diagnosed dengue haemorrhagic fever case fatality rates. Trop Doct. 2003 Jul;33(3):156-9. [PubMed]
12. Badave GK, P. Sai Swaroop, P. Nageswara Rao. Importance of NS1 antigen detection and its association with platelet count for early diagnosis of dengue virus infection. Int. J. Curr. Microbiol. App. Sci 2015;4(3):779- 84.
13. Kulkarni RD, Patil SS, Ajantha GS, Upadhya AK, Kalabhavi AS, Shubhada RM, Shetty PC, Jain PA. Association of platelet count and serological markers of dengue infection- importance of NS1 antigen. Indian J Med Microbiol. 2011 Oct-Dec;29(4):359-62. doi: 10.4103/0255-0857.90159. [PubMed]
14. Datta S, Wattal C. Dengue NS1 antigen detection: a useful tool in early diagnosis of dengue virus infection. Indian J Med Microbiol. 2010 Apr-Jun;28(2):107-10. doi: 10.4103/0255-0857.62484. [PubMed]
15. Shrivastava A, Dash PK, Tripathi NK, Sahni AK, Gopalan N, Lakshamana . Evaluation of commercial dengue NS1 enzyme-linked immunosorbent assay for early diagnosis of dengue infection. Indian J Med Microbiol 2011;29:51-56.
16. Sindhanai V, Sageera B, Rajkumar N, Suresh C, Evaluation of Correlation between Dengue Serological Markers and Platelet Count. Sch. J. App. Med. Sci., 2016; 4(2D):618-622.
17. Philippe B, Sutee Y, Rosanna W; Peeling, Elizabeth H; Laboratory Tests For The Diagnosis Of Dengue Virus Infection; Working paper for the Scientific Working Group on Dengue Research, convened by the Special Programme for Research and Training in Tropical Diseases, Geneva, 2006.
18. Golia S, Halli VH, Sujatha K, Karjigi, Reddy M, Kamath AS. Serodiagnosis of dengue using NS1 antigen, dengue IgM, dengue IgG antibody with correlation of platelet counts. International Journal of AJ Institute of Medical Sciences 2012; 1(2).112-117.
19. Ghosh K, Gangodkar S, Jain P, Shetty S, Ramjee S, Poddar P, Basu A. Imaging the interaction between dengue 2 virus and human blood platelets using atomic force and electron microscopy. J Electron Microsc (Tokyo). 2008 Jun;57(3):113-8. doi: 10.1093/jmicro/dfn007. Epub 2008 May 8. [PubMed]
20. Basu A, Jain P, Gangodkar SV, Shetty S, Ghosh K. Dengue 2 virus inhibits in vitro megakaryocytic colony formation and induces apoptosis in thrombopoietin-inducible megakaryocytic differentiation from cord blood CD34+ cells. FEMS Immunol Med Microbiol. 2008 Jun;53(1):46-51. doi: 10.1111/j.1574-695X.2008.00399.x. Epub 2008 Mar 25. [PubMed]
21. Rao SM. Correlation of clinical and laboratory parameters in dengue fever patients and its usefulness as bedside markers. Indian Journal of Critical Care Med. 2014;18(3):183-184. doi:10.4103/0972-5229.128713.
OAI - Open Archives Initiative
