Prevalence and antibiotic susceptibility pattern of Escherichia coli isolated from urine samples in patients attending a tertiary care hospital, Chennai
Keywords:
Escherichia coli, Urinary tract infection, Antibiotics, Resistance
Abstract
Background: Escherichia coli (E.coli)is one of the commonest cause of urinary tract infections. The drug resistance to many of the common antibiotics in use, has been alarming in the urinary E. coli isolates.
Objective: To study the prevalence of E.coli in urinary tract infections and to study their antibiotic susceptibility pattern of the isolates.
Material and Methods: This study was conducted in a tertiary care hospital in Chennai over a period of 4 months. A total of 3408 urine samples were processed for bacterial culture using standard methods. E.coli isolates were further confirmed by standard microbiological and biochemical methods. Antibiotic susceptibility of the E.coli isolates were carried out by Kirby Bauer disc diffusion method.
Results: A total of 102 E.coli isolates were obtained from the urine specimen and were screened for the antibiotic susceptibility pattern. E.coli isolates were highly susceptible to imipenem (100%) and nitrofurantoin (96%). High degree of resistance was seen to antibiotics like ampicillin, amoxycillin clavulanic acid, cefazolin and cotrimoxazole, ciprofloxacin and cefotaxime.
Conclusion: Our results give the data about the antibiotic susceptibility pattern of the Escherichia coli isolates in this locality. This will help in the choice of the appropriate antibiotics for treatment of the infections.
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References
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25. Sharifian M, Karimi A, Rafiee-Tabatabaei S, et al. Microbial sensitivity pattern in urinary tract infections in children: a single center experience of 1177 urine cultures. Jpn J Infect Dis. 2006; 59:380–382.
26. Kothari A, Sagar V. Antibiotic resistance in pathogens causing community-acquired urinary tract infections in India: a multicenter study. J Infect Dev Ctries. 2008 Oct 1;2(5):354-8.doi: 10.3855/jidc.196.
2. Daoud Z, Claude Afif. Escherichia coli Isolated from Urinary Tract Infections of Lebanese Patients between 2000 and 2009: Epidemiology and Profiles of Resistance.Chemotherapy Research and Practice Volume 2011 (2011), Article ID 218431, 6 pages http://dx.doi.org/10.1155/2011/218431.
3. RangarajSelvarangan, Pawel Goluszko, Jyotsana Singhal, Christophe Carnoy, Steve Moseley, Billy Hudson et al. Interaction of Dr Adhesin with Collagen Type IV Is a Critical Step in Escherichia coli Renal Persistence . Infection and Immunity. 2004;72(8):4827-4835. doi:10.1128/IAI.72.8.4827-4835.2004.
4. Gales, A. C., R. N. Jones, K. A. Gordon, H. S. Sader, W. W. Wilke, M. L. Beach, M. A. Pfaller, G. V. Doern, and the SENTRY Study Group (Latin America). 2000. Activity and spectrum of 22 antimicrobial agents tested against urinary tract infection pathogens in hospitalized patients in Latin America: report from the second year of the SENTRY antimicrobial surveillance program (1998). J. Antimicrob. Chemother 2000 Mar;45(3):295–303. [PubMed]
5. Gupta, K., T. M. Hooton, C. L. Wobbe, and W. E. Stamm. The prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in young women. Int. J. Antimicrob. Agents 1999 May;11(3-4):305–8.
6. Wiles TJ, Kulesus RR, Mulvey MA. Origins and virulence mechanisms of uropathogenic Escherichia coli. Exp Mol Pathol.2008Aug;85(1):11-9. doi: 10.1016/j.yexmp.2008.03.007. Epub 2008 Apr 8. [PubMed]
7. Daoud Z, Salem Sokhn E, Masri K, Cheaito K, Haidar-Ahmad N, Matar GM et al. Escherichia coli isolated from urinary tract infections of Lebanese patients between 2005 and 2012: epidemiology and profiles of resistance. Front. Med.2015;2:66. doi: 10.3389/fmed.2015.00026.
8. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL. Multidrug-resistant, extensivelydrug-resistant and pandrug-resistantbacteria: an internationalexpertproposal for interimstandarddefinitions for acquiredresistance.Clin Microbiol Infect.2012Mar;18(3):268-81. doi: 10.1111/j.1469-0691.2011.03570.x. Epub 2011Jul27. [PubMed]
9. Howard DH, Scott RD 2nd, Packard R, Jones D. The Global Impact of Drug Resistance. Clin Infect Dis 2003 Jan15;36(Suppl 1):S4-10 DOI:10.1086/344656. [PubMed]
10. Coates A, Hu Y, Bax R, Page C. The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov. 2002 Nov;1(11):895-910. [PubMed]
11. Robert Blomgran,Limin Zheng and OlleStendahl. Uropathogenic Escherichia Coli trigger oxygen-dependent apoptosis in human neutrophils through the cooperative effect of type I fimbriae and lipopolysaccharide. Infect Immun. 2004 Aug; 72(8): 4570–4578.doi: 10.1128/IAI.72.8.4570-4578.2004.
12. Jha, N and Bapat, S.K. A study of sensitivity and resistance of pathogenic microorganisms causing UTI in Kathmandu Valley. Kathmandu Univ.Med .J. 2005;3(10):123-129.
13. Karlowsky JA, Jones ME, Draghi DC, Thornsberry C, Sahm DF, Volturo GA. Prevalence and antimicrobialsusceptibilities of bacteriaisolated from blood cultures of hospitalizedpatients in the United States in 2002.Ann Clin Microbiol Antimicrob. 2004 May 10;3:7. [PubMed]
14. Aibinu, I.E., Adenipekun, E.A., Odugbemi, T.O. Emergence of Quinolone Resistance amongst E.coli Strains Isolated from Clinical Infections in Some Lagos State Hospitals, in Nigeria. Nigerian Journal of Health and Biomedical Sciences Vol.3(2) 2004: 73-78.
15. Sabir S, Ahmad Anjum A, Ijaz T, Asad Ali M, Ur Rehman Khan M, Nawaz M. Isolation and antibioticsusceptibility of E. coli from urinary tract infectionsin atertiary carehospital. Pak J Med Sci.2014 Mar;30(2):389-92.
16. Aziz Q, Ali Z, Izhar M, Shah VH. Antimicrobial resistance; comparison of Escherichia coli in different areas of Lahore. Professional Med J May-June 2012;19(3).
17. O Akingbade, S Balogun, D Ojo, P Akinduti, P O Okerentugba,J C Nwanze, et al. Resistant plasmid profile analysis of multidrug resistant Escherichia coli isolated from urinary tract infections in Abeokuta, Nigeria. Afr Health Sci. 2014 Dec; 14(4): 821–828.doi : 10.4314/ahs.v14i4.8.
18. Oteo J, Campos J, Baquero F; Spanish members of the European Antimicrobial Resistance Surveillance System. Antibiotic resistance in 1962 invasive isolates of Escherichia coli in 27 Spanish hospitals participating in the European Antimicrobial Resistance Surveillance System (2001). J Antimicrob Chemother. 2002 Dec;50(6):945-52. [PubMed]
19. Alos, J.I., J.L. Gómez-Garcés, I. García-Bermejo, J.J. García-Gómez, R. Gonzalez-Palacios and B. Padilla. The prevalence of Escherichia coli susceptibility to quinolone and other antibiotics in community-acquired bacteriurias in Madrid. Med. sClin., (Barc) 1993;101:8790.
20. Akinjogunla, O. J., Odeyemi, A. T. and Olasehinde,G. I. Epidemiological Studies of Urinary Tract Infection (UTI) among Post-menopausal Women in Uyo Metropolis, South-South, Nigeria. Journal of American Science 2010; 6(12):1674-1681.
21. O Akingbade, S Balogun, D Ojo, P Akinduti, P O Okerentugba, J C Nwanze et al. Resistant plasmid profile analysis of multidrug resistant Escherichia coli isolated from urinary tract infections in Abeokuta, Nigeria. Afr Health Sci. 2014 Dec; 14(4): 821–828.doi: 10.4314/ahs.v14i4.8.
22. Iqbal, M., I.K. Patel, Q. Ain,N.Barney,Q. Kiani,K.Z. Rabbani,G et al. Susceptibility Patterns of Escherichia coli: Prevalence of Multidrug- resistant Isolates and Extended Spectrum Beta-Lactamase Phenotype. J Pak Med Assoc. 2002 Sep;52(9):407-11.
23. Mavroidi A, Miriagou V, Liakopoulos A, Tzelepi Ε, Stefos A, Dalekos GN, Petinaki E. Ciprofloxacin-resistant Escherichia coli in Central Greece: mechanisms of resistance and molecular identification. BMC Infect Dis. 2012 Dec 23;12:371. doi: 10.1186/1471-2334-12-371. [PubMed]
24. Haque R, Akter ML, Salam MA. Prevalence and susceptibility of uropathogens: a recent report from a teaching hospital in Bangladesh. BMC Research Notes. 2015;8:416. doi:10.1186/s13104-015-1408-1.
25. Sharifian M, Karimi A, Rafiee-Tabatabaei S, et al. Microbial sensitivity pattern in urinary tract infections in children: a single center experience of 1177 urine cultures. Jpn J Infect Dis. 2006; 59:380–382.
26. Kothari A, Sagar V. Antibiotic resistance in pathogens causing community-acquired urinary tract infections in India: a multicenter study. J Infect Dev Ctries. 2008 Oct 1;2(5):354-8.doi: 10.3855/jidc.196.
CITATION
DOI: 10.17511/jopm.2017.i04.03
Published: 2017-12-31
How to Cite
Dr. Lakshmi K., Dr. Aishwarya J.R., Dr. Sharanya K., Dr. Sindhu Cugati, & Dr. Chitralekha S. (2017). Prevalence and antibiotic susceptibility pattern of Escherichia coli isolated from urine samples in patients attending a tertiary care hospital, Chennai. Tropical Journal of Pathology and Microbiology, 3(4), 376-381. https://doi.org/10.17511/jopm.2017.i04.03
Section
Original Article
OAI - Open Archives Initiative
